Solution cytology fixatives employed in ether / alcohol 96 to equally present in disuse because of the dangerous characteristics of the 96 th OL ether is used most often, the procedure is based on the preparation immersed in the bath for a minimum of 10 to 15 min Other alcohols such as 100% methanol, propanol 80% and isopropanol 80% Citospray is used as in the samples obtained by forced peeling q sample types can reach a cytology laboratory samples that can reach the lab from samples obtained by AP are forced exfoliation by rubbing or scraping with various instruments. Is applied to the skin and organs accessible from the outside exfoliation cytology samples forced through liq spontaneously transporting elements scaly (urine, sputum) through liq through examination (endoscopy) fine needle aspiration (FNA) of lesions organs without direct access yq no spontaneous secretion and sputum samples FNA special outlines the procedure d a urine sample in the AP service is fixing to send in a bottle and fixed with 50% alcohol before 12 o'clock for suspension Spin cel in the cytospin of 10 'at 1500 rpm decanted to remove supernatant liq Homogenization Extención Fixing with OL 96 or demonstration of citospray stain Meto2 PAS Reaction carbohydrate 1 cn HIO ₄ oxidation reaction of the aldehyde formed with the Formation of Schiff coloration rojomagenta PAS positive polysaccharides Carbohydrates simple (liver) neutral mucopolysaccharides (stomach colon bacterial capsule) mucoproteins (lungs, thyroid) glycoproteins glycolipids (SNC) pigments (ceroid lipofuscin) Carbohydrates negative PAS PAS acid mucopolysaccharides 2 Meto2 amylase amylases catalyze the hydrolysis of ls glycosidic bonds of starch, glycogen and glycogen PAS PASA - / neutral mucopolysaccharide glycoprotein PAS PASA + / no-go-carbohydrate PAS PAS techniques to identify false positives based on d block functional groups and is based negativity dl d the PAS reaction after d 1.2 d acetylation glycol groups present and the aldehyde group HC d Existence Without a completed block aldehyde groups preesistente d can u q the result is false (+) In this case, undertake a control tissue not oxidized and then one rusting and compares the result 3 cationic dye-based Technique for HC acidsCornflower Blue cn combination used in the method of PAS acid GAG (blue) and neutral glycoproteins GAG (magenta) d toluidine blue metachromasia Grups cromotopos acids (red) dl tej rest (blue) ls Method spicer diamines reactive agent oxidanre + ( Cl ₃ Fe) stains sulfated GAG alone and combined with alcian blue distinguishes neutral d ls GAG GAG acidic colloidal iron method ls pull tissue acid groups set the Fe ^{3 +} and this is demonstrated later by the Prussian blue reaction of 4 Technique methenamine silver ls q carbonyl radicals come from the oxidation of HC ls reduce silver salts that produce q metallic silver precipitate on the structures in MB q Positive staining with PAS-positive glycoproteins